Identification and quantification of 5,6,7,8‐tetrahydro‐2‐(2‐phenylethyl)chromones in Chinese eaglewood by HPLC with diode array detection and MS |
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| Authors: | Yong Jiang Qian Zhang Liang Zhang Peng‐Fei Tu |
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| Institution: | 1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, , Beijing, P. R. China;2. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, , Beijing, P. R. China |
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| Abstract: | A sensitive and reliable HPLC coupled with diode array detection and MS method was developed and validated for the first time to simultaneously identify and quantify eight characteristic 5,6,7,8‐tetrahydro‐2‐(2‐phenylethyl)chromones (THPECs) in Chinese eaglewood. Chromatographic separation was performed on a Zorbax SB C18 column with a gradient of acetonitrile/0.1% formic acid/water as the mobile phase. The MS fragmentation behavior of THPECs was characterized as the successive neutral loss of two molecules of H2O (M+H–18–18]+) and then two molecules of CO (M+H–18–18–28–28]+), which could be used to differentiate Chinese eaglewood from counterfeits. Validation of the developed analytical method showed good linearity, satisfactory precision, and good recovery. The established method was successfully applied to the simultaneous determination of eight THPECs in ten batches of Chinese eaglewood, which could be used as a tool for the quality control of Chinese eaglewood. |
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| Keywords: | Aquilaria sinensis Chinese eaglewood Chromones Quality control |
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