Quantification of taxanes in a leaf and twig extract from Taxus baccata L. using 13C NMR spectroscopy |
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Authors: | Mathieu Paoli Ange Bighelli Vincent Castola Félix Tomi Joseph Casanova |
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Affiliation: | Université de Corse, UMR CNRS 6134 SPE, Equipe Chimie et Biomasse, , 20000 Ajaccio, France |
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Abstract: | In the course of our ongoing work on the chemical characterization of Taxus baccata L. growing wild in Corsica, we have developed and validated a method for direct quantification of taxane derivatives by 13C NMR using 10‐deacetylbaccatin III as reference compound and 1,6‐hexanediol as internal standard. We have observed good accuracy (relative errors between 0.3% and 3.5%), linearity (R2 = 0.999) and precision (reproducibility 8.5 mg ± 1.1%) of the measurements. The experimental procedure was applied to the quantification of six identified taxanes in a fraction of chromatography of a methanol extract of T. baccata leaves. This method can be applied to other compounds bearing the taxane skeleton. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | 13C NMR quantification taxanes Taxus baccata L |
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