Systematic assignment of NMR spectra of 5‐substituted‐4‐thiopyrimidine nucleosides |
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| Authors: | Xiaohui Zhang Jian Wang Yao‐Zhong Xu |
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| Institution: | 1. College of Environment and Chemical Engineering, Dalian University, , Dalian, 116622 China;2. Department of Life, Health and Chemical Sciences, The Open University, , Milton Keynes, MK7 6AA UK |
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| Abstract: | Unambiguous characterization of 5‐substituted‐4‐thiopyrimidine nucleosides (ribonucleosides and 2'‐deoxynucleosides) was performed using NMR spectroscopy. Assignments of all proton and carbon signals of 5‐bromo‐4‐thiouridine and related nucleosides were systematically carried out and firmly established by COSY and HMQC techniques. The NMR data of various 4‐thiopyrimidine nucleosides are compared, and the key contributing factors discussed. The approach presented here is applicable to other modified nucleosides and nucleotides, as well as nucleobases. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | NMR 1H 13C 5‐substituted pyrimidine 4‐thiothymidine 4‐thiouridine 5‐bromo‐4‐thiouridine nucleoside DNA RNA |
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