Ni(II)‐NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV‐Derived Peptides
1. Leibniz‐Institut für Polymerforschung Dresden e.V., Hohe Stra?e 6, D‐01069 Dresden, Germany;2. Organische Chemie der Polymere, Technische Universit?t Dresden, D‐01062 Dresden, Germany;3. Molecular ImmunoBiology Laboratory, Hospital General Gregorio Mara?on, c/Dr. Esquerdo 46, E‐28007 Madrid, Spain;4. Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, D‐01069 Dresden, Germany
Abstract:
Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)‐nitrilo(triacetic acid)‐modified poly(ethylene imine)‐maltose ( Ni‐NTA‐DG ) is realized and evaluated as complexation agent against His‐tagged peptides using fluorescence polarization and dynamic light scattering. The polyplexes are stable until a pH of 6.5–6.0, and also up to 50 mM of imidazole. A first uptake approach shows that polyplexes lead to an increase in peptide uptake in monocyte‐derived immature dendritic cells. In summary, Ni‐NTA‐DG represents a promising (delivery) platform for forthcoming in vitro applications.
