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Selectivity tuning via temperature pulsing using low thermal mass liquid chromatography and monolithic columns |
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| Authors: | Matthias Pursch Patric Eckerle Binghe Gu Jim Luong Hernan J Cortes |
| Institution: | 1. Dow Deutschland Anlagengesellschaft mbH & OHG, Analytical Technology Center, , Rheinmuenster, Germany;2. The Dow Chemical Company, Analytical Sciences, , Midland, MI, USA;3. Dow Canada, Analytical Technologies, , Fort Saskatchewan, Alberta, Canada;4. Hernan J. Cortes Consulting LLC, , Midland, MI, USA;5. University of Tasmania, , Hobart, Australia |
| Abstract: | Low thermal mass LCwas applied to the capillary LC separation of a complex insecticide mixture by increasing temperature and decreasing gradients, as well as fast selected temperature pulses to increase resolution of overlapped components. The technology was applied using a new generation of capillary monolithic stationary phases. Considerable peak shifts and selectivity changes were observed for given temperature conditions. The concept of temperature pulsing during an elution profile shows promise for increasing resolution in difficult separations and can provide a relatively simple means to solve coelution problems. |
| Keywords: | Low‐thermal mass LC Monolith Temperature gradient |