Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high‐speed counter‐current chromatography |
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Authors: | Hui‐Lan Yue Xiao‐Hui Zhao Li‐Juan Mei Yun Shao |
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Affiliation: | 1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, , Xining, China;2. College of Life Sciences, University of Chinese Academy of Sciences, , Beijing, China |
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Abstract: | Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high‐speed counter‐current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two‐phase solvent system composed of ethyl acetate/n‐butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27 mg of forsythoside B, 41 mg of verbascoside, 29 mg of alyssonoside, 23 mg of isoverbascoside, and 13 mg of leucosceptoside B with purities of 97.7, 99.2, 99.5, 99.3, and 97.3%, respectively, were obtained in a one‐step separation within 4 h from 150 mg of crude extract. The recoveries of the five PhGs after MR‐HSCCC separation were 74.5, 76.5, 72.5, 76.4, and 77.0%, respectively. The chemical structures of all five compounds were identified by 1H and 13C NMR spectroscopy. |
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Keywords: | HSCCC Lamiophlomis rotata (Benth.) Kudo Macroporous resins Phenylpropanoid glycosides Traditional Chinese medicines |
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