| 喜树碱与胰蛋白酶的相互作用 |
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| 引用本文： | 贾旭,邓珊珊,李政,苏波,刁家志,刘芯韵,郑芸,刘克武.喜树碱与胰蛋白酶的相互作用[J].化学研究与应用,2006,18(12):1408-1412. |
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| 作者姓名： | 贾旭 邓珊珊 李政 苏波 刁家志 刘芯韵 郑芸 刘克武 |
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| 作者单位： | 1. 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川,成都,610064
2. 江南大学生物工程学院,江苏,无锡,214036 |
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| 摘 要： | 喜树碱(camptothecin,CPT)是从珙桐科乔木喜树中分离得到的一类重要抗癌药物,对动物肿瘤及白血病均有明显的抑制作用1]。CPT分子为五环结构,含有一个吡咯3,4-b]喹啉环,一个共轭吡啶环和一个α-羟基六元内脂环2]。CPT通过嵌合抑制DNA拓扑异构酶Ⅰ的活性,对卵巢癌、结肠直肠癌
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| 关 键 词： | 喜树碱 胰蛋白酶 紫外光谱 荧光淬灭 |
| 文章编号： | 1004-1656（2006）1408-05 |
| 收稿时间： | 2005-02-15 |
| 修稿时间： | 2005-06-02 |
Interaction between Trypsin and Camptothecin |
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| JIA Xu,DENG Shan-shan,LI Zheng,SU Bo,Diao Jia-zhi,LIU Xin-yun,ZHENG Yun,LIU Ke-wu.Interaction between Trypsin and Camptothecin[J].Chemical Research and Application,2006,18(12):1408-1412. |
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| Authors: | JIA Xu DENG Shan-shan LI Zheng SU Bo Diao Jia-zhi LIU Xin-yun ZHENG Yun LIU Ke-wu |
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| Institution: | 1. College of Life Science,Sichuan University,Key Laboratory of Bio-resources and Eco-environment,Ministry of Education,Chengdu 610064,China;2.School of Biotechnology,Southem Yangtze University,Wuxi 214036,China |
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| Abstract: | The interaction of trypsin with camptothecin(CPT) in vitro was studied by ultraviolet(UV) absorption spectral and fluorescence spectral methods.Making out value of Ki according to the ratio between 1/v and the amount of inhibitor contributes to the conclusion that CPT is a noncompetitive inhibitor.The interaction between CPT and trypsin is quite strong.CPT can affect the conformation of trypsin in some degree.Fluorescence quenching contributes to nonradiative energy-transfer,which results a static quenching of CPT to trypsin.Their binding constants and the binding sites of CPT were determined. |
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| Keywords: | camptothecin trypsin ultraviolet spectrum fluorescen quenching |
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