Extraction and Structure Investigation of the Poly-saccharides from the Pollen of Typha Angustifolia L.

Three polysaccharides, TAA, TAB, TAC have been isolated from the pollen of Typha angustifolia L. , and purified by hot water extraction, alcohol precipitation, Sevage method, gel filtration (Sephadex G50) and ion-exchange chromatography on DEAE Sephadex A25 and DEAE-cellulose. Their purities were tested by gel filtration and electrophoresis. Their structures were characterized by paper chromatography of the hydrolysis products, GLC analysis of the alditol acetates, alkylation analysis, 1H NMR and 13C NMR spectra. TAA is mainly composed of a-L-arabinofuanose, β-D-galactose and α-D-galacturonic acid, while the backbones of TAB and TAC are composed of (1→5)-linked α- L-arabinofuranosyl residues.

Extraction and Structure Investigation of the Poly-saccharides from the Pollen of Typha Angustifolia L.