A study of glutamine interference in the acidified ninhydrin reaction for proline |
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| Authors: | Yosh Kimura |
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| Institution: | Washington State University, Western Washington Research and Extension Center, Puyallup, Wash.U.S.A. |
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| Abstract: | In the application of the acidified ninhydrin reaction to the estimation of free proline in plant extracts, glutamine was a major source of interference, all others being minor, absent or removable with Permutit. Some characteristics of glutamine interference in this reaction are described. A ratio of linear errors method for the correction of proline values to the glutamine free state is introduced. The glycine and proline concentration in the reaction mixtures are prime factors in obtaining a ratio of linear errors by spectrophotometric measurement at 2 wavelengths. |
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