A Novel Method for Screening G‐quadruplex Stabilizers to Human Telomeres |
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Authors: | Jen‐Fei Chu Zi‐Fu Wang Ting‐Yuan Tseng Ta‐Chau Chang |
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Institution: | 1. Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan, R.O.C.;2. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan, R.O.C.;3. Department of Chemistry, National Taiwan University, Taipei, Taiwan, R.O.C.;4. Institute of Biophotonics, National Yang‐Ming University, Taipei, Taiwan, R.O.C. |
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Abstract: | We present a simple method based on the Cu2+ induced unfolding of G‐quadruplex (G4) of human telomere sequence dAG3(T2AG3)3] to screen a number of 3,6‐bis(1‐methyl‐4‐vinylpyridinium)carbazole diiodide (BMVC) analogues for better G4 stabilizers. Using circular dichroism (CD), the screening results suggest that the tri‐cations of 9‐substituted BMVC derivatives are better G4 stabilizers than the bi‐cations of BMVC. In addition, 3,6‐bis(1‐methyl‐4‐vinylpyrazinium)carbazole diiodide (BMVC4) is likely a better core molecule than BMVC for G4 stabilizers. |
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Keywords: | G‐quadruplex Stabilizer BMVC derivatives Copper induced unfolding |
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