The determination of chromium in biological materials by atomic absorption spectroscopy |
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| Authors: | Fredric J. Feldman Edward C. Knoblock William C. Purdy |
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| Affiliation: | Division of Biochemistry, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington., D.C. 20012 U.S.A.;University of Maryland, College Park. Md.U.S.A. |
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| Abstract: | Biological samples were mineralized with a mixture of nitric, sulfuric, and perchloric acids. The chromium was oxidized to chromium(VI) followed by extraction into methyl isobutyl ketone in the cold. The ketone extract was aspirated into a fuel-rich hydrogen-air flame, and the absorption recorded using the 357.9 nm line of chromium. The limit of detection for chromium was found to be 10 p.p.b. Tissue chromium levels of normal albino rats were determined. Increased chromium values were observed for animals maintained on a high chromium diet compared to those fed a deficient diet. |
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