In vivo resolution of conflicting in vitro results: synthesis of biotin from dethiobiotin does not require pyridoxal phosphate |
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| Authors: | Abdel-Hamid Ahmed M Cronan John E |
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| Affiliation: | Department of Microbiology, University of Illinois, Urbana, IL 61801, USA. |
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| Abstract: | The source of the biotin sulfur atom remains a contested point in studies of biotin synthase (BioB) in vitro. Recent reports that BioB has an intrinsic pyridoxal phosphate (PLP)-dependent cysteine desulfurase activity were tested by depleting Escherichia coli cells of PLP. The BioB-dependent conversion of dethiobiotin to biotin proceeded in these cells irrespective of the presence or absence of PLP. |
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