用胶束电动毛细管色谱法分离黄芩中黄酮类有效成分

应用胶束电动毛细管色谱技术分离黄芩中主要的黄酮类有效成分并进行分析，确定它们分别是：黄芩甙、黄芩素、汉黄芩甙、汉黄芩素、木蝴蝶素Ａ及木蝴 Ａ－７－Ｄ－葡萄糖甙。缓冲液由２０ｍｍｏｌ／ＬＳＤＳ、２０ｍｍｏｌ／ＬＬ的磷酸三氮钠及２５ｍｍｏｌ／Ｌ的硼砂组成，溶液的Ｐ来７．２４。在胶束电动车管色谱分析中，黄芩中的主要黄酮类有铲成分在１５ｍｉｎ内即可达到基线分离，并且能对黄芩甙进行定量分析，笔者还讨论了表面

STUDY ON MAJOR FLAVONOIDS IN CRUDE SCUTELLABIAE RADIX BY MICELLAE ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

A method,of micellar electrokinetic capillary electrophoresis for determining the six flavonoids in scutellariae radix,i.e.,baiealin,baicalein,wogonin 7-O-glneuronide,wogonin,oroxyiin A-7-O-glucuronide and oroxyiin A,was developed,A buffer solution (pH 7.24) was composed of 20 mmol/L sodium dodecyl sulphate (SDS),20 mmol/L sodium dihydrogenphosphate and 25 mmol/L sodium borate was found to be the most suitable electrolyte for die separation.The contents of the six flavonoids in crude sculellariae radix could easily be determined within alxmt 15 min.On-column UV (254 nm) monitoring allowed quantity determination of baicalin.The effects of pH.surfactant concentration and applied voltage on the migration behavior of the solutes were studied.