| 钴卟啉的合成及消除有毒氧自由基的研究 |
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| 引用本文： | 李早英,张悦宁,李聪,冯清,夏淑贞. 钴卟啉的合成及消除有毒氧自由基的研究[J]. 武汉大学学报(理学版), 2000, 46(2): 150-152 |
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| 作者姓名： | 李早英 张悦宁 李聪 冯清 夏淑贞 |
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| 作者单位： | 1. 武汉大学化学与环境科学学院武汉 430072
2. 武汉同济医科大学化学教研室武汉 430030 |
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| 基金项目： | 国家自然科学基金资助项目(29972035);湖北省自然科学基金资助项目(98J043) |
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| 摘 要： | 合成与结构表征了4个钴卟啉配合物Co(Por),Por=5,10,15,20-四[(3,4,5-三甲氧基苯基)卟啉(1),(对甲基苯基)卟啉(2),(对氯苯基)卟啉(3),(对磺酸基苯基)卟啉(4)].并用它们作为超氧化物歧化酶(SOD)和过氧化氢酶(CAT)模型物进行了双功能模拟酶活性的测试核黄素-蛋氨酸光照法证实,在10-5～10-6mol/L浓度范围内4种配合物均有消除超氧自由基((O-2)的作用;分光光度法证实具有催化H2O2的分解作用,其分解率随浓度的增大而增大;用小鼠肝匀浆法测定,四种钴卟啉均表明有良好的抗脂质过氧化作用.
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| 关 键 词： | 钴卟啉 合成 超氧化物歧化酶 过氧化氢酶 双功能模拟酶 |
| 文章编号： | 0253-9888(2000)02-0150-03 |
| 修稿时间： | 1999-06-20 |
Study on Synthesis and Deplete Harmful Radical of Cobalt Porphyrins as the Mimic of Enzyme |
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| LI Zao|ying ,ZHANG Yue|ning ,LI Cong ,FENG Qing ,XIA Shu|zhen. Study on Synthesis and Deplete Harmful Radical of Cobalt Porphyrins as the Mimic of Enzyme[J]. JOurnal of Wuhan University:Natural Science Edition, 2000, 46(2): 150-152 |
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| Authors: | LI Zao|ying ZHANG Yue|ning LI Cong FENG Qing XIA Shu|zhen |
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| Affiliation: | LI Zao|ying 1,ZHANG Yue|ning 1,LI Cong 1,FENG Qing 2,XIA Shu|zhen 2 |
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| Abstract: | The four cobalt porphyrins [Co(3,4,5|MeO|TPP)(1); Co(TTP)(2); Co(4|Cl|TPP)(3); Co(4|HSO 3|TPP)(4)] were synthesized and considered as two mimic of superoxide dismutaes(SOD) and catalase (CAT). The catalytic efficiency of them on deplete the harmful radical have been proved using riboflavin|methionine photoreduction method in the concentration range of 10 -6 ~10 -5 mol/L. The catalytic efficiency of them at decomposing H 2O 2 has been performed by spectrophotometer. The percentage of decomposing H 2O 2 while the increasing the concentration of the imitating compounds. The lipid peroxidation produced in liver homogenated of mice was determined by spectrophotometer detecting the content of MDA. |
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| Keywords: | cobalt porphyrin synthesis superoxide dismutaes catalase two functional mimic of enzyme |
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