珊瑚来源群体淬灭活性产生菌的筛选与抑菌活性研究

珊瑚病原菌的群体感应（Quorum Sensing，QS）是介导珊瑚疾病发生的重要因素，抑制病原菌的群体感应可以有效抵抗病原菌的侵染作用。为解决耐药性难题并维持珊瑚礁生态系统健康，本研究以紫色杆菌（Chromobacterium violaceum）为指示菌，从三亚市鹿回头半岛三亚湾（109°28''E，18°13''N）采集的珊瑚样品中，分离筛选具有较高群体淬灭酶活性的珊瑚共附生细菌，经16S rDNA测序初步鉴定其大部分为弧菌属。进一步研究群体淬灭活性菌株对两株珊瑚致病菌溶珊瑚弧菌V545（Vibrio coralliilyticus）和溶藻弧菌Z-14（V.alginolyticus）的生长、运动及生物被膜生成的影响。结果显示，5株群体淬灭活性菌株明显抑制溶珊瑚弧菌V545及溶藻弧菌Z-14的生长，8个菌株对溶藻弧菌Z-14的运动有明显抑制作用；9个菌株可显著抑制溶珊瑚弧菌V545生物被膜形成，13个菌株可显著抑制溶藻弧菌Z-14的生物被膜形成，其中11个菌株对溶藻弧菌Z-14生物被膜生长抑制率均超过85%；部分群体淬灭活性菌株对溶珊瑚弧菌V545和溶藻弧菌Z-14的生长、运动及生物被膜形成均有显著抑制作用。本研究筛选获得具有群体淬灭活性的细菌，并揭示其群体淬灭活性对珊瑚致病菌生长、运动、生物膜生成的影响，为解决珊瑚微生物抗病机制提供理论依据与原创材料。

Screening of Quorum Quenching Bacterial Strains from Coral and Analysis of Its Bacteriostatic Activity

The quorum sensing (QS) of coral pathogens is an important factor that mediates the occurrence of coral diseases,inhibiting pathogens can effectively resist the infection of pathogens.In order to solve the problem of drug resistance and maintain the health of the coral reef ecosystem,in this research,Chromobacterium violaceum was used as indicator bacteria,the coral symbiotic bacteria with high colony quenching enzyme activity were isolated and screened from the coral samples collected from Luhuitou fringing reef in Sanya Bay (109°28''E,18°13''N).16S rDNA sequencing preliminarily identified most of them as Vibrio alginolyticus.The effect of fermentation supernatant on the growth,movement and the biofilm formation of two coral pathogenic bacteria V.coralliilyticus V545 and V.alginolyticus Z-14 were studied further.The results showed that the growth of V.coralliilyticus V545 and V.alginolyticus Z-14 were significantly inhibited by 5 strains of QS activity,and the movement of V.alginolyticus Z-14 was significantly inhibited by 8 strains.9 strains could significantly inhibit the biofilm formation of V.coralliilyticus V545,13 strains could significantly inhibit the biofilm formation of V.alginolyticus Z-14,among which 11 strains inhibited the growth of biofilm formation of V.alginolyticus Z-14 up to 85%.Some groups of quenched active strains have a significant inhibitory effect on the growth,movement and biofilm formation of V.coralliilyticus V545 and V.alginolyticus Z-14.This study screened and obtained bacteria with colony quenching activity,and revealed the effects of colony quenching activity on the growth,movement,and biofilm formation of coral pathogenic bacteria,providing theoretical basis and original materials for solving the mechanisms of coral disease resistance.