UHPLC/Q-Orbitrap-MS结合化学计量学方法研究酸枣仁入血成分

建立了一种基于超高效液相色谱-质谱联用技术结合化学计量学快速分析酸枣仁入血成分的方法。以腹腔注射对氯苯丙氨酸(PCPA)建立失眠大鼠模型,给药组连续灌胃给予酸枣仁水提物(30 g/kg)5 d后,分别收集模型组和给药组的血清样品。采用Oasis PRIME HLB 96孔板对血清样品预处理,以超高效液相色谱-静电场轨道离子阱质谱(UHPLC/Q-Orbitrap-MS)进行数据采集,最后采用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)及变量投影重要性分析(VIP)筛选差异性的原型化合物及代谢产物。共鉴定和推断了20个入血成分,包括11个原型成分及9个代谢产物。研究结果为进一步深入探讨酸枣仁改善失眠的效应物质提供了基础。

Analysis of Absorbed Components in Rat Serum after Oral Ziziphi Spinosae Semen by UHPLC/Q-Orbitrap-MS Combined with Chemometrics

An ultra high liquid chromatography-quadrupole orbitrap mass spectrometry(UHPLC/Q-Orbitrap-MS)combined with chemometrics was developed for the rapid analysis of absorbed components of Ziziphi Spinosae Semen in rat serum.A model for insomnia rat was established by intraperitoneal injection of para-chlorophenylalanine(PCPA).After intragastric administration of Ziziphi Spinosae Semen aqueous extract for five successive days,the serum samples from the model group and the Ziziphi Spinosae Semen aqueous extract treatment group were collected.The protein was removed using Oasis PRIME HLB 96-well plates.The data was acquired by UHPLC/Q-Orbitrap-MS,and analyzed by chemometrics including principal component analysis(PCA),orthogonal partial least-squares discriminant analysis(OPLS-DA)and variable importance in the projection(VIP)to screen different prototype compounds and metabolites.A total of 20 components were identified,including 11 prototype components and 9 metabolites.The developed method provides a basis for further study on the effective substances of Ziziphi Spinosae Semen for insomnia improvement.