Binding and Sensing Properties of a Hybrid Naphthalimide–Pyrene Aza-Cyclophane towards Nucleotides in an Aqueous Solution |
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Authors: | Aleksandr M Agafontsev Aleksandr S Oshchepkov Tatiana A Shumilova Evgeny A Kataev |
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Institution: | 1.N. N. Vorozhtsov Institute of Organic Chemistry SB RAS, 9th Lavrentiev Avenue, 630090 Novosibirsk, Russia;2.Institute of Chemistry, Technische Universität Chemnitz, 09107 Chemnitz, Germany; (A.S.O.); (T.A.S.);3.Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Nikolaus-Fiebiger-Str. 10, 91058 Erlangen, Germany |
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Abstract: | Selective recognition of nucleotides with synthetic receptors is an emerging direction to solve a series of nucleic acid-related challenges in biochemistry. Towards this goal, a new aza-cyclophane with two different dyes, naphthalimide and pyrene, connected through a triamine linker has been synthesized and studied for the ability to bind and detect nucleoside triphosphates in an aqueous solution. The receptor shows Foerster resonance energy transfer (FRET) in fluorescence spectra upon excitation in DMSO, which is diminished dramatically in the presence of water. According to binding studies, the receptor has a preference to bind ATP (adenosine triphosphate) and CTP (cytidine triphosphate) with a “turn-on” fluorescence response. Two separate emission bands of dyes allow one to detect nucleotides in a ratiometric manner in a broad concentration range of 10−5–10−3 M. Spectroscopic measurements and quantum chemical calculations suggest the formation of receptor–nucleotide complexes, which are stabilized by dispersion interactions between a nucleobase and dyes, while hydrogen bonding interactions of nucleobases with the amine linkers are responsible for selectivity. |
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Keywords: | cyclophane nucleotide recognition supramolecular recognition host– guest chemistry fluorescent probe |
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