CG base pair recognition by substituted phenylimidazole nucleosides |
| |
Authors: | Wang Wei Purwanto Maria G M Weisz Klaus |
| |
Institution: | Institut für Chemie, Freie Universit?t Berlin, Germany. |
| |
Abstract: | Four nonnatural imidazole nucleosides with different substituents were synthesized and studied for their binding to a CG Watson-Crick base pair by NMR spectroscopic techniques in an aprotic solvent. Concentration and temperature dependent measurements allowed the determination of association constants, association enthalpies and entropies. Strong binding was observed with analogues carrying an ureidophenyl substituent and corresponding enthalpies of association are compatible with the anticipated formation of three hydrogen bonds to the CG base pair. In contrast, only weak binding was observed for analogues with an aminophenyl or benzamidophenyl substituent. 2D NOE measurements at low temperatures confirm the proposed binding mode for the high-affinity ligands but indicate binding interactions for the weakly bound analogues different from the expected geometry. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|