Rapid serotyping of enteropathogenic Yersinia Enterocolitica strains by co-agglutination |
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Institution: | 1. Department of Microbiological Diagnostics & Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark;2. Department of Microbiology & Infection Control, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen, Denmark;3. Department of Production and Development, SSI Diagnostica, Herredsvejen 2, 3400 Hillerød, Denmark |
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Abstract: | A rapid co-agglutination test using monospecific antisera was developed for the serological typing of enteropathogenic strains of Yersinia enterocolitica. A total of 70 bacterial strains (17 reference strains and 53 clinical isolates) were examined. Absorption of immune sera against srotypes O:3, O:8 and O:9 with their heterologous antigens (S-LPS) was necessary to avoid the appearance of different cross-reactions, as observed by co-agglutination. The proteins present in the S-LPS preparations obtained from each serotype seemed to be responsible for such cross-reactions. Results obtained with a total of 57 clinical isolates belonging to other members of the family Enterobacteriaceae indicated a high specificity of the assay. |
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