Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography |
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| Authors: | Myrdal Paul B Angersbach B Steven Karlage Kelly Kuehl Philip J |
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| Institution: | University of Arizona, College of Pharmacy, 1703 East Mabel Street, Tucson, AZ 85721, USA. myrdal@pharmacy.arizona.edu |
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| Abstract: | An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 degrees C. |
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