Electrochemical oxidation and determination of ceftriaxone on a glassy carbon and carbon-nanotube-modified glassy carbon electrodes |
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Authors: | S Majdi A Jabbari H Heli H Yadegari A A Moosavi-Movahedi S Haghgoo |
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Institution: | (1) Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, P.O. Box: 16315-1618, Tehran, Iran;(2) Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;(3) Center of Quality Control of Drug, Tehran, Iran |
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Abstract: | The electrochemical behavior of ceftriaxone was investigated on a carbon-nanotube-modified glassy carbon (GC-CNT) electrode
in a phosphate buffer solution, pH = 7.40, and the results were compared with those obtained using the unmodified one glassy
carbon (GC) electrode]. During oxidation of ceftriaxone, an irreversible anodic peak appeared, using both modified and unmodified
electrodes. Cyclic voltammetric studies indicated that the oxidation process is irreversible and diffusion-controlled. The
number of electrons exchanged in the electrooxidation process was obtained, and the data indicated that ceftriaxone is oxidized
via a one-electron step. The results revealed that carbon nanotube promotes the rate of oxidation by increasing the peak current.
In addition, ceftriaxone was oxidized at lower potentials, which thermodynamically is more favorable. These results were confirmed
by impedance measurements. The electron-transfer coefficients and heterogeneous electron-transfer rate constants for ceftriaxone
were reported using both the GC and GC-CNT electrodes. Furthermore, the diffusion coefficient of ceftriaxone was found to
be 2.74 × 10−6 cm2 s−1. Binding of ceftriaxone to human serum albumin forms a kind of electroreactive species. The percentage of interaction of
ceftriaxone with protein was also addressed. A sensitive, simple, and time-saving differential-pulse voltammetric procedure
was developed for the analysis of ceftriaxone, using the GC-CNT electrode. Ceftriaxone can be determined with a detection
limit of 4.03 × 10−6 M with the proposed method. |
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Keywords: | Ceftriaxone Carbon nanotube Human serum albumin Electrocatalysis Differential pulse voltammetry |
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