Fast liquid chromatography combined with mass spectrometry for the analysis of metabolites and proteins in human body fluids |
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Authors: | Linda Kortz Christin Helmschrodt Uta Ceglarek |
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Institution: | (1) Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, Liebigstrasse 27, 04103 Leipzig, Germany; |
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Abstract: | In the last decade various analytical strategies have been established to enhance separation speed and efficiency in high
performance liquid chromatography applications. Chromatographic supports based on monolithic material, small porous particles,
and porous layer beads have been developed and commercialized to improve throughput and separation efficiency. This paper
provides an overview of current developments in fast chromatography combined with mass spectrometry for the analysis of metabolites
and proteins in clinical applications. Advances and limitations of fast chromatography for the combination with mass spectrometry
are discussed. Practical aspects of, recent developments in, and the present status of high-throughput analysis of human body
fluids for therapeutic drug monitoring, toxicology, clinical metabolomics, and proteomics are presented. |
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Keywords: | |
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