Molecular site assessment and process monitoring in bioremediation and natural attenuation |
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Authors: | Gary S Sayler Alice Layton Curtis Lajoie John Bowman Mike Tschantz James T Fleming |
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Institution: | (1) The Center for Environmental Biotechnology, The University of Tennessee, 37922 Knoxville, TN |
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Abstract: | A variety of modern biotechnical approaches are available to assist in optimizing and controlling bioremediation processes.
These approaches are broad-ranging, and may include genetic engineering to improve biodegradative performance, maintenance
of the environment, and process monitoring and control. In addition to direct genetic engineering strategies, molecular diagnostic
and monitoring technology using DNA gene probing methods and new quantitative mRNA analytical procedures allows direct analysis
of degradative capacity, activity, and response underin situ conditions. Applications of these molecular approaches in process developments for trichloroethylene (TCE), polychlorinated
biphenyls (PCB), and polynuclear aromatic hydrocarbons (PAH) bio-oxidation in soils, aquifer sediments, and ground-water treatment
reactors have been demonstrated. Molecular genetic technologies permit not only the development of new processes for bioremediation,
but also new process monitoring, control strategies, and molecular optimization paradigms that take full advantage of vast
and diverse abilities of microorganisms to destroy problem chemicals. |
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Keywords: | Bioremediation environmental biotechnology trichloroethylene polychlorinated biphenyls polynuclear aromatic hydrocarbons |
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