Simultaneous and sensitive determination of procaine and its metabolite for pharmaceutical quality control and pharmacokinetic research by using a graphite paste electrode |
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Authors: | Ai-Lin Liu Jia-Dong Wang Wei Chen Xing-Hua Xia Yuan-Zhong Chen Xin-Hua Lin |
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Institution: | (1) Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350004, People’s Republic of China;(2) Fujian Institute of Hematology, The Affiliated Union Hospital of Fujian Medical University, Fuzhou, 350001, China;(3) State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, People’s Republic of China; |
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Abstract: | A simple, rapid, sensitive, and accurate method for simultaneous electrochemical determination of procaine and its metabolite
(p-aminobenzoic acid, PABA) for pharmaceutical quality control and pharmacokinetic research was developed using a graphite paste
electrode. The differential pulse voltammetric results revealed that procaine and p-aminobenzoic acid, respectively, showed well-defined anodic oxidation peaks on a carbon paste electrode with a current peak
separation of 155 mV at a scan rate of 100 mV s−1. This well separation of the current peaks for these two compounds in voltammetry enables us to simultaneously determine
them. Good linearity (r > 0.998) between oxidation peak current and concentration was obtained in the range of 5.0 × 10−7–5.0 × 10−5 M for procaine and 5.0 × 10−7–2.0 × 10−5 M for PABA in pH 4.50 acetate buffer solution. The detection limit for both analytes is 5 × 10−8 M (S/N = 3:1). The present voltammetric method has been successfully used to determine trace p-aminobenzoic acid in procaine hydrochloride injection and procaine in plasma with a linear relationship of current to its
concentration ranging from 1.0 × 10−6 to 5.0 × 10−5 M (correlation coefficient of 0.9981) with a low detection limit of 5.0 × 10−7 M (S/N = 3:1). This validated method is promising to the study of pharmacokinetics in Sprague–Dawley rat and rabbit plasma after
an intravenous administration of procaine hydrochloride injection. |
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