Multispectroscopic DNA interaction studies of a water-soluble nickel(II) complex containing different dinitrogen aromatic ligands |
| |
| Authors: | Nahid Shahabadi Soheila Kashanian Marzieh Khosravi Maryam Mahdavi |
| |
| Institution: | (1) Department of Inorganic Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran;(2) Faculty of Chemistry, Sensor and Biosensor Research Center and Nanoscience and Nanotechnology Research Center, Razi University, Kermanshah, Iran |
| |
| Abstract: | The water-soluble Ni(II) complex, Ni(bipy)2(phen-dione)](OAc)2·2H2O (bipy = 2,2′-bipyridine and phen-dione = 1,10-phenanthroline-5,6-dione) has been synthesized and characterized by physico-chemical
and spectroscopic methods. The binding interactions of this complex with calf thymus DNA (CT-DNA) were investigated using
fluorimetry, spectrophotometry, circular dichroism and viscosimetry. In fluorimetric studies, the enthalpy and entropy of
the reaction between the complex and CT-DNA showed that the reaction is exothermic (ΔH = −123.9 kJ mol−1; ΔS = −323.5 J mol−1 K−1). The competitive binding studies showed that the complex could not release methylene blue completely. The complex showed
absorption hyperchromism in its UV–Vis spectrum with DNA. The calculated binding constant, K
b obtained from UV–Vis absorption studies was 2 × 105 M−1. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA, as well as changes in its viscosity. The
results suggest that this nickel(II) complex interact with CT-DNA via a groove-binding mode. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
