胆管癌新鲜离体组织的傅里叶变换红外光谱研究

该研究探讨了运用傅里叶变换红外(FTIR)光谱技术对胆管癌进行术中原位、无创和快速诊断的可行性。联合应用傅里叶变换红外光谱仪和衰减全反射(ATR)探头，测定了26例胆管癌和43例良性胆管疾病新鲜离体组织的红外吸收光谱，发现每条光谱在3 800～1 000 cm-1之间循序出现了12个谱带。测量各个谱带的峰位、峰强和峰面积值，然后计算出各谱带的相对峰强和相对峰面积比值，最后进行标准统计学分析。比较胆管癌与良性胆管组织的光谱发现有以下特征：(1)2 925 cm-1谱带明显地向低波数移位(P=0.033);(2)峰强比I_{1 083}/I_{1 460}(P=0.005)和峰面积比S1 083/S_{1 460}(P=0.001)，S_{1 240}/S_{1 460}(P=0.025)明显升高，说明癌组织中核酸相对脂类含量明显增加;(3)峰强比I_{1 550}/I_{1 083}(P=0.000)和峰面积比S_{1 550}/S_{1 083}(P=0.000)明显下降，提示蛋白质相对核酸的含量下降。研究结果表明，FTIR有望成为一种术中原位、在体和快速诊断胆管癌的新方法。

Investigation on Fourier Transform Infrared Spectrum of Freshly Resected Cholangiocarcinoma Tissue

To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared(FTIR)spectroscopy,26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery.And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection(ATR)without pretreatment immediately after resection.For each spectrum,12 bands were identified and assigned between 3 800 and 1 ...