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| PDMS微芯片吸附牛血清白蛋白的研究 | |
| 引用本文： | 刘春叶,张剑,许旭.PDMS微芯片吸附牛血清白蛋白的研究[J].化学研究与应用,2009,21(5). |
| 作者姓名： | 刘春叶 张剑 许旭 |
| 作者单位： | 1. 西安医学院药学系,陕西,西安,710021;西安交通大学生命科学与技术学院,陕西,西安,710049 2. 西安第二炮兵工程学院503教研室,陕西,西安,710025 3. 中国科学院上海有机化学研究所,上海,2000321 |
| 摘 要： | 材料表面的物理和化学性质对蛋白质的吸附具有很大的影响1].对蛋白质吸附的研究是研制生物传感器、生物芯片和生物材料的基础. |
| 关 键 词： | 聚二甲基硅氧烷 蛋白质 吸附 |
Study of the adsorption of bovine serum albumin on PDMS microchip |
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| LIU Chun-ye,ZHANG Jian,XU Xu.Study of the adsorption of bovine serum albumin on PDMS microchip[J].Chemical Research and Application,2009,21(5). | |
| Authors: | LIU Chun-ye ZHANG Jian XU Xu |
| Institution: | 1.School of Pharmacy;Xi'an Medical University;Xi'an 710021;China;2.Xi'an Research Institute of Hi-Tech.Hongqing Town;Xi'an 710025;3.Shanghai Institute of Organic Chemistry;Chinese Academy of Sciences;Shanghai 200032;4.School of Life Science and Technology;Xi'an Jiaotong University;Xi'an 710049;China |
| Abstract: | The adsorption of protein on poly dimethylsiloxane(PDMS) which often used to make microchip was studied in this paper.Effects of the pH and concentration of buffer solution,three surfactant concentration,and time on the adsorption of bovine serum albumin(BSA) were investigated in detail.The absorbency of BSA was measured with the ultraviolet spectrophotometer.The results showed that the adsorption of BSA reached minimum when buffer solution pH was 7.0 and concentration was 40 mmol/L.The suppression of prote... |
| Keywords: | poly dimethylsiloxane protein adsorption |
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