Analysing the isotopic life history of the alpine ungulates Capra ibex and Rupicapra rupicapra rupicapra through their horns

The horn of ungulate grazers offers a valuable isotopic record of their diet and environment. However, there have been no reports of the spatio‐temporal variation of the isotopic composition of horns. We investigated patterns of carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopic composition along and perpendicular to the horn axis in Capra ibex and Rupicapra rupicapra rupicapra to assess the effects of animal age, within‐year (seasonal) and inter‐annual variation, natural contamination and sampling position on horn isotope composition. Horns of male C. ibex (n = 23) and R. r. rupicapra (n = 1) were sampled longitudinally on the front (only R. r. rupicapra) and back side and on the surface and sub‐surface. The sides of the R. r. rupicapra horn did not differ in δ¹³C. In both species, the horn surface had a 0.15‰ lower δ¹³C and a higher carbon‐to‐nitrogen (C/N) ratio than the sub‐surface. Washing the horn with water and organic solvents removed material that caused these differences. With age, the δ¹⁵N of C. ibex horns increased (+0.1‰ year^?1), C/N ratio increased, and ¹³C discrimination relative to atmospheric CO₂ (¹³Δ) increased slightly (+0.03‰ year^?1). Geostatistical analysis of one C. ibex horn revealed systematic patterns of inter‐annual and seasonal ¹³C changes, but ¹⁵N changed only seasonally. The work demonstrates that isotopic signals in horns are influenced by natural contamination (δ¹³C), age effects (¹³Δ and δ¹⁵N), and seasonal (δ¹³C and δ¹⁵N) and inter‐annual variation (δ¹³C). The methods presented allow us to distinguish between these effects and thus allow the use of horns as isotopic archives of the ecology of these species and their habitat. Copyright © 2009 John Wiley & Sons, Ltd.