Analysis of impurities in streptomycin and dihydrostreptomycin by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time‐of‐flight mass spectrometry |
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Authors: | Shin‐ichi Kawano |
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Institution: | Analytical Applications Department, Shimadzu Corporation, 1 Nishinokyo‐Kuwabaracho, Nakagyo‐ku, Kyoto 604‐8511, Japan |
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Abstract: | Impurities in streptomycin (STR) and dihydrostreptomycin (DHS) were investigated by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time‐of‐flight mass spectrometry (HILIC/ESI‐QIT/TOFMS). Samples were separated on a fused‐core silica column (100 mm × 2.1 mm i.d., particle size: 2.7 µm) with isocratic elution using 200 mM ammonium formate buffer (pH 4.5) and acetonitrile as mobile phase. Constant neutral loss survey in accurate mass measurement was carried out by QIT/TOFMS. Formulae, chemical structures of impurities in an STR sample were suggested with supporting results on the probable pathways of STR biosynthesis by Streptomyces griseus. Copyright © 2009 John Wiley & Sons, Ltd. |
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