Sensitivity-enhanced 2D IPAP,TROSY-anti-TROSY,and E.COSY experiments: alternatives for measuring dipolar 15N-1HN couplings |
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Authors: | Ding Keyang Gronenborn Angela M |
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Institution: | Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 130, Bethesda, MD 20892-0520, USA. |
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Abstract: | Sensitivity-enhanced versions of the IPAP, TROSY-anti-TROSY, and E.COSY experiments for measuring one-bond 15N-1HN couplings are presented. Together with the previously developed sensitivity-enhanced E.COSY-type HSQC experiment they comprise a suite of sensitivity-enhanced experiments that allows one to chose the optimal spectrum for accurate measurement of one-bond 15N-1HN residual dipolar couplings in proteins. Since one-bond 15N-1HN residual dipolar couplings play uniquely important roles in structural NMR, these additional methods provide further tools for improving structure determination of proteins and other biological macromolecules. |
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Keywords: | Residual dipolar couplings IPAP TROSY– anti-TROSY E COSY Sensitivity enhancement |
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