免疫亲和柱净化、在线电化学衍生化高效液相色谱法检测花生中的黄曲霉毒素

 采用免疫亲和柱净化、在线电化学衍生化高效液相色谱法测定了花生中黄曲霉毒素（AFT）B 1，B2，G1和G2。以体积分数为80%的甲醇提取样品中的AFT，经免疫亲和柱净化洗脱 后，以Kobra Cell装置在线衍生，反相HPLC分离定量。4种毒素的分离在13 min内完成，检 出限均达到0.1 μg/kg。5次测定花生样品的RSD值为9.2%～15%；样品添加标样0.5 ～9.0 μg/kg，回收率为74.8%～97.3%。

Determination of Aflatoxins in Peanut by High Performance Liquid Chromatograp hy Using Immunoaffinity Column Clean-Up and On-Line Electrochemical Deriva tization

A method for the determination of aflatoxin B1, B2, G1 and G2 in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B1 and G1 were derivatized with on-line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 microgram/kg for each toxin. The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2%-15% and the average recovery of AFT spiked at 0.5-9.0 micrograms/kg levels was in the range of 74.8%-97.3%.