环糊精毛细管区带电泳分离手性药物对映体

分别以2种天然环糊精(β、γ-环糊精)、2种常用的电中性环糊精衍生物(羟丙基-β-环糊精、二甲基-β-环糊精)和3种新型荷电环糊精衍生物(高取代磺酸基α、β、γ-环糊精)作为毛细管区带电泳手性添加剂,研究了环糊精的类型对6种手性药物对映体分离的影响.2种天然环糊精对所研究的手性药物均无手性识别能力,而环糊精经过衍生化后手性识别能力得到了很大的提髙,尤其是高取代磺酸基β-环糊精使6种手性药物均得到了基线分离.还考察了缓冲溶液的pH值和有机添加剂对手性分离的影响.

Enantioseparation of Chiral Drugs by Cyclodextrin-Mediated Capillary Zone Electrophoresis

The effects of the cyclodextrin (CD) type on the enantioseparation of six chiral drugs were investigated, using 2 natural CD, namely β-CD and γ-CD, 2 commonly used uncharged functionalized CD, namely hydroxypropylated β-CD (HP-β-CD) and dimethylated β-CD (DM-β-CD), and 3 newly-developed charged CD, namely highly sulfated α-CD (HS-α-CD), highly sulfated β-CD (HS-β-CD), highly sulfated γ-CD (HS-γ-CD) as capillary zone electrophoresis additive, respectively. While the 2 natural CD showed no enantioselectivity of the 6 chiral drugs, the functionalized CD's enantioselectivity improved greatly. In particular, the HS-β-CD could baseline chirally resolved the enantiomers of all the 6 chiral drugs, indicating that the HS-β-CD is a versatile chiral selector and is worth developing further. The effects of the pH of the background electrolyte and the organic modifier on the enantioseparation were also examined and disscussed.