Protein fractionation and detection for metalloproteomics: challenges and approaches |
| |
Authors: | Barnett James P Scanlan David J Blindauer Claudia A |
| |
Institution: | (1) Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK;(2) School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK; |
| |
Abstract: | At least one third of all proteins are thought to require a metal ion co-factor for their function. Recognition of the importance
of metals in biological systems and major advances in analytical instrumentation and technology have led to the emergence
of the new research area of metalloproteomics in recent years. Despite this progress, the experimental determination of in-vivo
metal cofactors has remained challenging, because this requires elucidation of protein interactions with non-covalently bound
metal ions. This critical review highlights current methodological approaches, focusing, in particular, on issues relating
to the fractionation and separation of the metalloproteome, including recent experience with metalloproteomics for marine
cyanobacteria in our laboratory. Metalloproteomics promises to deliver novel insights into fundamental biological processes
in the future, but it is clear that further methodological advances are necessary to exploit the full potential of this emerging
research area. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|