Improved method for fatty acid analysis in herbage based on direct transesterification followed by solid-phase extraction |
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Authors: | Alves Susana P Cabrita Ana R J Fonseca António J M Bessa Rui J B |
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Institution: | REQUIMTE, Unidade de Produ??o Animal, Instituto Nacional de Recursos Biológicos, Fonte-Boa, 2005-048 Vale de Santarém, Portugal. |
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Abstract: | Direct transesterification (DT) and solvent extraction with acid or basic derivatization procedure for fatty acid (FA) analysis in herbage were compared. The highest total FA, alpha-linolenic and linoleic acid contents were obtained with DT. However, DT also produced the highest amount of interfering compounds, identified as phytadienes and sugar derivative products, which may co-elute with FA. An additional step based on solid-phase extraction to produce clean samples was proposed. This procedure is simple and gives good recoveries for the FA fortified samples. Additionally, structural characterization of 16:1 trans-3 was conducted by covalent adduct chemical ionization tandem mass spectrometry. |
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