ADP-specific sensors enable universal assay of protein kinase activity |
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| Authors: | Srinivasan Jayaram Cload Sharon T Hamaguchi Nobuko Kurz Jeffrey Keene Sara Kurz Markus Boomer Ryan M Blanchard Jill Epstein David Wilson Charles Diener John L |
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| Affiliation: | Archemix Corporation, 1 Hampshire Street, Cambridge, MA 02139, USA. |
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| Abstract: | Two molecular sensors that specifically recognize ADP in a background of over 100-fold molar excess of ATP are described. These sensors are nucleic-acid based and comprise a general method for monitoring protein kinase activity. The ADP-aptamer scintillation proximity assay is configured in a single-step, homogeneous format while the allosteric ribozyme (RiboReporter) sensor generates a fluorescent signal upon ADP-dependent ribozyme self-cleavage. Both systems perform well when configured for high-throughput screening and have been used to rediscover a known protein kinase inhibitor in a high-throughput screening format. |
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