Preparation and application of polymer-grafted magnetic nanoparticles for lipase immobilization |
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| Authors: | Yang Yong Yongxiao Bai Yanfeng Li Lei Lin Yanjun Cui Chungu Xia |
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| Affiliation: | 1. State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering & Environmental Technology, Lanzhou University, Lanzhou 730000, China;2. Institute of Materials Science and Engineering, Lanzhou University, Lanzhou 730000, China;3. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract: | Functionalized superparamagnetic particles were prepared by graft polymerization of glycidyl methacrylate and methacryloxyethyl trimethyl ammonium chloride onto the surface of modified-Fe3O4 nanoparticles. The resultant particles were characterized by X-ray powder diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and vibrating sample magnetometry. The results indicate that the polymer chains had been effectively grafted onto the surface of Fe3O4 nanoparticles. The functionalized particles remained dispersive and superparamagnetic. Lipase was immobilized on the magnetic particles under mild conditions by electrostatic adsorption and covalent binding with the activity recovery up to 70.4%. The immobilized lipase had better thermal stability compared to free lipase. |
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| Keywords: | Fe3O4 nanoparticle Polymerization Functionalized Immobilized lipase |
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