Acrosome reaction in Octopus tankahkeei induced by calcium ionophore A23187 and a possible role of the acrosomal screw |
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| Authors: | Zhe Li Jun-Quan Zhu Wan-Xi Yang |
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| Institution: | 1. The Sperm Laboratory, College of Life Sciences, Zhejiang University, 388 Yu Hang Tang Road, Hangzhou, Zhejiang 310058, China;2. Faculty of Life Sciences and Biotechnology, Ningbo University, Zhejiang 315211, China;1. Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland;2. Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland;3. Department of Histology and Embryology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland;4. Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Doktora Judyma 24, 71-466 Szczecin, Poland;5. Department of General Biology and Parasitology, Medical University of Warsaw, Cha?ubinskiego 5, 02-004 Warsaw, Poland;6. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Swiecickiego 6, 60-781 Poznan, Poland;1. Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25 Vodňany, Czech Republic;2. Veterinary Biomedical Sciences Department, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada;3. Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada;4. Department of Ichthyobiology and Fisheries, University of Agriculture, Kraków 30-199, Poland;5. Research Unit Animal and Functionalities of Animal Products, INRA, University of Lorraine, Vandoeuvre-lès-Nancy 54505, France;6. Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, 165 21 Prague 6, Czech Republic;1. The Sperm Laboratory, College of Life Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China;2. The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China;1. College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China;2. Bio-Transduction Lab, Wuhan Institute of Biotechnology, 666 Gaoxin Road, Wuhan East Lake High-tech Development Zone, Wuhan, China |
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| Abstract: | The octopod sperm is unique especially in two aspects: the screw-shaped acrosome and its inner layered substructure (striation). The present study aims to investigate morphological changes of Octopus tankahkeei spermatozoa during the acrosome reaction (AR) and to pursue functions of the internal substructures revealed by inducing AR with the calcium ionophore A23187. Gradual changes of the spermatozoa were traced using fluorescence and electron microscopy. The AR process included the bulging, vesiculation, and dehiscence of the plasma membrane around the acrosome and the nucleus, as well as the vesiculation of the mitochondrial sheath. Membrane vesiculation outside the nucleus has never been reported in the order Octopoda. The rigid screw and the inner striation of the acrosome remained intact surmounting the nucleus, suggesting that these two structures have potential functions during fertilization. In addition, the detachment of the sperm head and the tail was commonly observed in this study, both in intact and acrosome-reacted sperm. Fluorescence microscopy revealed that the detached mitochondrial sheath usually gave weaker and more dispersive signals than the joint ones. This phenomenon implied that the intense energy release might promote the detachment of the mitochondrial sheath. |
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