| 一种快速制备毕赤酵母表达系统P CR鉴定模板DNA的方法 |
| |
| 引用本文： | 任耀军,孙慧兰,颜赛勋. 一种快速制备毕赤酵母表达系统P CR鉴定模板DNA的方法[J]. 新疆师范大学学报(自然科学版), 2014, 0(4): 27-29 |
| |
| 作者姓名： | 任耀军 孙慧兰 颜赛勋 |
| |
| 作者单位： | 1. 新疆干旱区湖泊环境与资源重点实验室，新疆 乌鲁木齐830054; 新疆师范大学 实验室与设备管理处，新疆 乌鲁木齐830054
2. 新疆干旱区湖泊环境与资源重点实验室,新疆 乌鲁木齐,830054 |
| |
| 基金项目： | 新疆自治区自然科学基金，新疆干旱区湖泊环境与资源重点实验室基金(XJDX0909-2013-01)资助。 |
| |
| 摘 要： | 文章研究了简便高效获取毕赤酵母表达系统PCR鉴定模板DNA的提取方法，采用酶消化和物理冻熔结合的方法，提取的酵母基因组DNA直接PCR扩增检测质量。结果表明，该方法提取酵母基因组DNA具有质量高、成本低、耗时短、操作简单等优点。
|
| 关 键 词： | 毕赤酵母 基因组DNA 快速制备 PCR扩增 |
A Method for Rapid Preparing DNA Template to Identify Pichia pastoris Expression System by PCR Amplification |
| |
| REN Yao-jun,SUN Hui-lan,YAN Sai-xun. A Method for Rapid Preparing DNA Template to Identify Pichia pastoris Expression System by PCR Amplification[J]. Journal of Xinjiang Normal University(Natural Sciences Edition), 2014, 0(4): 27-29 |
| |
| Authors: | REN Yao-jun SUN Hui-lan YAN Sai-xun |
| |
| Affiliation: | REN Yao-jun,SUN Hui-lan,YAN Sai-xun(1. Laboratory of Lake Environment and Resources in Arid Zone of Xinjiang, Urumqi, Xinjiang, 830054, China;2. The Laboratory ＆ Equipment Management Department of Xinfiang Normal University, Urumqi , Xinfiang , 830054, China) |
| |
| Abstract: | In order to obtain a simple and effective method for rapid preparing DNA template to identify Pichia pastoris expression system by PCR amplification, The method of enzyme digestion was combined application with Physics of Freezing ＆ Thawing to extract Pichia pastoris genome DNA, which was detected by PCR as template. The results showed that this method presented advantages of high quality extracts, lower cost, less time consuming and ease of operation. |
| |
| Keywords: | Pichia pastoris Genome DNA Rapid Preparing PCR Amplification |
| 本文献已被 维普 等数据库收录！ |
