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Sensitization of spinal cord nociceptive neurons with a conjugate of substance P and cholera toxin |
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| Authors: | Robert M Caudle Andrew J Mannes Jason Keller Federico M Perez Shelby K Suckow John K Neubert |
| Institution: | (1) Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, University of Florida College of Dentistry, Gainesville, FL 32610, USA;(2) Department of Orthodontics, , Gainesville, FL 32610, USA;(3) Department of Neuroscience, University of Florida College of Medicine, McKnight Brain Institute, Gainesville, FL 32610, USA;(4) Pain and Neurosensory Mechanisms Branch, National Institutes of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA |
| Abstract: | BackgroundSeveral investigators have coupled toxins to neuropeptides for the purpose of lesioning specific neurons in the central nervous system. By producing deficits in function these toxin conjugates have yielded valuable information about the role of these cells. In an effort to specifically stimulate cells rather than kill them we have conjugated the neuropeptide substance P to the catalytic subunit of cholera toxin (SP-CTA). This conjugate should be taken up selectively by neurokinin receptor expressing neurons resulting in enhanced adenylate cyclase activity and neuronal firing. |
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