含芘荧光化学敏感器化合物同小牛胸腺DNA分子间的相互作用

研究了含芘荧光化学敏感器分子被ctDNA猝灭的荧光光谱.ctDNA分子对该化学敏感器中芘的激发单体,激基缔合物都有猝灭作用.对激发单体的猝灭速度顺序为;化合物(2)>化合物(1)>芘丁酸>化合物(3);对激基缔合物的猝灭速度顺序为;化合物(2)化合物(3).由得到的荧光猝灭数据,可按公式(2)求得荧光化学敏感器分子与ctDNA分子相互作用的稳定常数.发现化合物(2)与ctDNA分子间有着最强的相互作用能力.按ctDNA和含芘荧光化学敏感器的分子结构、构型以及分子内原子-原子的间距等提出了ctDNA分子与该荧光化学敏感器的作用模型,并对上述结果进行了初步解释.

Interaction Between Flourescence Chemosensors with Pyrene and Calf Thymus DNA

The quenching spectra of fluorescence chemosensors with pyrene by calf thymus DNA were studied in this work. The fluorescence quenching of both monomer and excimer of pyrene by ctDNA were investigated. The quenching constants of fluorescence chemosensors by ctDNA from SternVolmer equation were compared as following: for monomer, compound 2>compound 1>pyrenyl burytic acid>compound 3; for excimer, compound 2compound 3 . The stability constants of the interaction between fluorescence chemosensors and ctDNA were calculated according to equation (2). Of which the interaction between fluorescence chemosensors and ctDNA has been proposed according to their molecular structure, configuration, atomatom distances and molecular size. The results obtained were discussed preliminarily.