Suppression of artifacts induced by homonuclear decoupling in amino-acid-type edited methyl H–C correlation experiments |
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Authors: | Hlne Van Melckebeke Jean-Pierre Simorre Bernhard Brutscher |
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Institution: | Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A.-UJF 41, rue Jules Horowitz, 38027 Grenoble Cedex, France |
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Abstract: | A detailed theoretical and experimental analysis of the artifacts induced by homonuclear band-selective decoupling during CT frequency labeling is presented. The effects are discussed in the context of an amino-acid-type editing filter implemented in 1H–13C CT-HSQC experiments of methyl groups in proteins. It is shown that both Bloch–Siegert shifts and modulation sidebands are efficiently suppressed by using additional off-resonance decoupling as proposed by Zhang and Gorenstein J. Magn. Reson. 132 (1998) 81], and appropriate adjustment of a set of pulse sequence parameters. The theoretical predictions are confirmed by experiments performed on 13C-labeled protein samples, yielding artifact-free amino-acid-type edited methyl spectra. |
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Keywords: | Amino-acid-type editing Bloch– Siegert shifts Hadamard spectroscopy Homonuclear decoupling Methyl NMR Proteins Resolution enhancement Sidebands |
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