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High-performance liquid chromatographic determination of major organic acids in apple juices and ciders
Authors:D Blanco Gomis  M J Moran Gutierrez  M D Gutierrez Alvarez  A Sanz Medel
Institution:(1) Departamento de Quimica Fisica y Analitica, Facultad de Quimica, Universidad de Oviedo, Oviedo, Spain
Abstract:Summary A reversed-phase high-pressure liquid chromatographic method is presented for the simultaneous separation and determination of malic, citric, lactic, succinic and ascorbic acids in apple juices and ciders. After filtration and/or degasification, the organic acids in the sample are separated on a LiChrosorb/C18 column and quantified by using a rapid diode array detector. The method is considered to be a suitable choice for the accurate and precise determination (C.V. 5%) of these compounds.
Keywords:High-performance liquid chromatography  Reversed-phase  Organic acids  Apple juice  Cider
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