Radiolabeling of Pluronic amphiphilic copolymer for adsorption studies |
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Authors: | De Cupere V M Biltresse S Marchand-Brynaert J Rouxhet P G |
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Affiliation: | Université Catholique de Louvain, Unite de Chimie des Interfaces, Croix du Sud 2/18, 1348 Louvain-la-Neuve, Belgium. |
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Abstract: | A new pathway for the radiolabeling of Pluronic PE6800 was developed. In a first step, the CH(2)-OH end groups of the copolymer were substituted by tosylates; in a second step these were reduced by [3H]-NaBH(4) to obtain tritiated chain ends. The final product was shown to be a mixture of native, tosylated, and reduced Pluronic containing 1 tritium atom per 1110 Pluronic molecules. The labeling procedure did not affect the molecular weight distribution nor the adsorption isotherm of the copolymer on polystyrene plates. A plateau value of about 0.7 microg/cm(2) is reached at a concentration in solution of 500 microg/ml, i.e., much lower than the cmc. Upon drying, the Pluronic adsorbed layer reorganizes in particles with a size of about 30 to 60 nm which cover about 15% of the substratum surface. This observation is of great importance for the design of protein-resistant surfaces by adsorption of Pluronic. |
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