| Affiliation: | (1) Key Lab for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, Peoples' Republic of China;(2) School of Life Science, Shandong University, Jinan, Shandong, Peoples' Republic of China;(3) Department of Chemistry, Jinan University, Jinan, Shandong, Peoples' Republic of China |
| Abstract: | A new quantitative method for micro amounts of nucleic acids in aqueous solution is proposed using Eu3+-benzoylacetone (BA) complex as fluorescent probe in the presence of cetyltrimethyl-ammonium bromide (CTMAB). Under the optimum condition, the ratio of the fluorescence intensities with and without nucleic acids is proportional to the concentration of nucleic acid in the range of 1.0 × 10−9 to 5.0 × 10−6 g/mL for herring sperm DNA (hsDNA), 3.0 × 10−9 to 1.0 × 10−6 g/mL for calf thymus DNA(ctDNA) and 8.0 × 10−9 to 1.0 × 10−6 g/mL for yeast RNA (yRNA), and their detection limits are 0.33, 0.21 and 0.99 ng/mL, respectively. Actual sample (DNA of Arabidopsis thaliana) was determined satisfactorily. In addition, the interaction mechanism is also investigated. |
