| 玻璃纤维素壳聚糖膜固定乙酰胆碱酯酶的研究 |
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| 引用本文： | 曲慧,车育,姜爱莉,于贞.玻璃纤维素壳聚糖膜固定乙酰胆碱酯酶的研究[J].烟台大学学报(自然科学与工程版),2013(3):178-181. |
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| 作者姓名： | 曲慧 车育 姜爱莉 于贞 |
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| 作者单位： | 烟台大学海洋学院,山东烟台264005 |
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| 基金项目： | 山东省自然科学基金资助项目（ZR2011DL008,Y2008D48） |
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| 摘 要： | 以玻璃纤维素壳聚糖膜为载体,戊二醛为交联剂,进行乙酰胆碱酯酶(AChE)的固定,并对固定化酶的理化性质进行研究.结果表明,乙酰胆碱酯酶的较佳固定化条件为:150 U/mL AChE液50μL,体积分数为5%的戊二醛溶液50μL,质量分数为1%的BSA100μL,pH值为8.0的0.2 mol/L PBS缓冲液,配制成1 mL酶液,玻璃纤维素壳聚糖膜浸于此酶液4℃固定8 h.固定化酶的最适作用温度37℃,最适pH 8.0,能够重复利用4次以上,表明该固定化酶的稳定性较高.
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| 关 键 词： | 玻璃纤维素壳聚糖膜 乙酰胆碱酯酶 固定化 |
Immobilization of Acetylcholinesterase with Chitosan Coated Glass Fibre Membrane |
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| QU Hui,CHE Yu,JIANG Ai-li,YU Zhen.Immobilization of Acetylcholinesterase with Chitosan Coated Glass Fibre Membrane[J].Journal of Yantai University(Natural Science and Engineering edirion),2013(3):178-181. |
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| Authors: | QU Hui CHE Yu JIANG Ai-li YU Zhen |
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| Institution: | (School of Ocean, Yantai University, Yantai 264005, China) |
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| Abstract: | Acetylcholinesterase is immobilized on chitosan coated glass fibre membrane using glutaraldehyde as cross-linking reagent. The benificial immobilizing conditions are obtained as follows： 1 mL of enzyme solution is prepared with 0. 2 mol/L phosphate buffer（pH 8.0） adding 50 μL 150 U/mL AChE solution, 50 μL 5% （V/V） glutaraldehyde and 100 μL 1% （W/W） BSA. The chitosan coated glass fibre membrane is immersed in the enzyme solution for 8 h at 4 ℃. The optimum temperature and pH value of immobilized AChE are 37 ℃ and 8.0, and the immobilized AChE could be reused four times. |
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| Keywords: | chitosan coated glass fibre membrane acetylcholinesterase immobilization |
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