| 液相色谱-串联质谱法测定饲料原料中26种霉菌毒素 |
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| 引用本文： | 王瑞国,苏晓鸥,程芳芳,王培龙,樊霞,张维.液相色谱-串联质谱法测定饲料原料中26种霉菌毒素[J].分析化学,2015(2):264-270. |
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| 作者姓名： | 王瑞国 苏晓鸥 程芳芳 王培龙 樊霞 张维 |
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| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所，农业部农产品质量安全研究重点实验室，北京100081 |
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| 基金项目： | “十二五”农村领域国家科技计划(No.2011BAD26B0405)资助This work was supported by the National Science & Technology Program in Rural Areas during the 12th Five-year Plan Period |
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| 摘 要： | 建立了同时检测玉米、豆粕等饲料原料中脱氧雪腐镰刀菌烯醇等26种霉菌毒素的液相色谱-串联质谱(LC-MS/MS)方法。玉米和豆粕样品经乙腈-水-甲酸(84∶15.9∶0.1,V/V)超声提取1 h,取1 m L上清液经Mycospin 400多功能净化柱净化,浓缩干燥,0.25 m L水-甲醇-甲酸(95∶4.9∶0.1,V/V)复溶,上机测定。采用反相C18色谱柱分离,以0.1%甲酸-水和0.1%甲酸-甲醇溶液作为流动相进行梯度洗脱,采用多反应监测离子模式进行定性分析。基质效应考察发现,样品提取液经Mycospin 400多功能净化柱净化后,大部分毒素仍有较强的基质效应。因此,采用基质匹配标准曲线外标法进行定量分析。在高、中、低3种添加浓度水平下,26种霉菌毒素的平均回收率为61.9%~119.5%,相对标准偏差介于0.8%~18.6%之间。针对不同目标物,本方法的定量限为0.5~25μg/kg。
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| 关 键 词： | 液相色谱-串联质谱 饲料原料 多功能净化柱 霉菌毒素 |
Determination of 26 Mycotoxins in Feedstuffs by Multifunctional Cleanup Column and Liquid Chromatography-Tandem Mass Spectrometry |
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| WANG Rui-Guo,SU Xiao-Ou,CHENG Fang-Fang,WANG Pei-Long,FAN Xia,ZHANG Wei.Determination of 26 Mycotoxins in Feedstuffs by Multifunctional Cleanup Column and Liquid Chromatography-Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2015(2):264-270. |
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| Authors: | WANG Rui-Guo SU Xiao-Ou CHENG Fang-Fang WANG Pei-Long FAN Xia ZHANG Wei |
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| Institution: | WANG Rui-Guo;SU Xiao-Ou;CHENG Fang-Fang;WANG Pei-Long;FAN Xia;ZHANG Wei;Institute of Quality Standards and Testing Technology for Agricultural Products,Chinese Academy of Agricultural Science,Key Laboratory of Agrifood Safety and Quality,Ministry of Agriculture; |
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| Abstract: | |
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| Keywords: | Liquid chromatography-tandem mass spectrometry Feedstuff Multifunctional cleanup column Mycotoxins |
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