空气动力辅助离子化质谱组织涂片分析用于胶质瘤异柠檬酸脱氢酶基因状态的快速判别

异柠檬酸脱氢酶(IDH)基因分型对于胶质瘤的精准诊疗具有重要意义。该文将组织涂片用于病理组织代谢物质谱分析的样品前处理,并采用2-羟基戊二酸与花生四烯酸质谱响应强度的比值作为胶质瘤IDH基因状态的判别指标,建立了基于空气动力辅助离子化(AFADESI)质谱技术的胶质瘤IDH基因状态快速判别分析方法。对组织涂片用于代谢物敞开式质谱分析的可行性进行考察和定量方法学验证,结果表明其具有样品分散性好、操作简单快速、代谢物覆盖度高、定量准确度好的优点。受试者工作曲线(ROC)评估显示,其用于胶质瘤IDH基因状态判别的诊断能力良好。组织涂片结合空气动力辅助离子化质谱分析用于胶质瘤IDH基因状态的快速判别有望为胶质瘤精准诊疗提供新的分析手段和技术支持。

Rapid Determination of Isocitrate Dehydrogenase Mutations in Human Glioma by Air Flow-assisted Desorption Electrospray Ionization Mass Spectrometry with Tissue Smears Pretreatment

Isocitrate dehydrogenase(IDH) genotype plays a significant role in precise diagnosis of glioma.An air flow assisted desorption electrospray ionization(AFADESI) mass spectrometry(MS) with rapid sample pretreatment using tissue smear instead of tissue cryo section was developed for rapid determination of the gene status of IDH in human glioma.Using intensity ratio of 2 hydroxyglutaric acid to arachidonic acid as an evaluation index,the IDH gene status of glioma tissue could be quickly identified.Quantitative evaluation as well as investigation on the feasibility for ambient mass spectrometry to analyze the metabolites confirmed that the tissue smear method was capable of rapid analysis of metabolites with high simplicity,high repeatability and high coverage.Receiver operating characteristic curve(ROC) demonstrated that the method possessed a strong diagnostic capacity for identifying the gene status of IDH in glioma.Therefore,the developed method could be expected to be a novel technique for precise diagnosis of glioma.