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Capillary electrophoresis with electrochemiluminescence detection of procyclidine in human urine pretreated by ion-exchange cartridge |
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| Authors: | Xiuhua Sun Jifeng Liu Weidong Cao Xiurong Yang Erkang Wang Y. S. Fung |
| Affiliation: | a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Renmin Street, 159 Jilin, Changchun 130022, China b Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China |
| Abstract: | This paper describe a Ru(bpy)32+ based electrochemiluminescence (ECL) method to detect procyclidine in human urine following separation by capillary electrophoresis (CE). An ECL detection cell was designed for post-column addition of Ru(bpy)32+. Parameters affecting separation and detection were optimized, leading to a detection limit of 1×10−9 mol/l in an on-capillary stacking mode. For application in urine, a cartridge packed with slightly acidic cation-exchange resin was used to eliminate the matrix effects of urine and improve the detection sensitivity. Extraction recovery was nearly 90%. |
| Keywords: | Capillary electrophoresis Electrochemiluminescence Procyclidine Ion-exchange cartridge |
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