北五味子中一种新的莽草酸衍生物的分离纯化及其含量测定

从北五味子中提取分离得到一种新的莽草酸衍生物, 经一维、二维核磁共振波谱(1D NMR, HMQC, HMBC)等数据解析, 鉴定为莽草酸正丁酯. 并采用ZORBAX Extend C18柱, 以乙腈-0.05%磷酸为流动相进行梯度洗脱, 检测波长为215 nm, 流速1.0 mL/min, 建立了北五味子中莽草酸正丁酯含量的高效液相色谱分析方法. 结果表明, 莽草酸正丁酯在2~65 μg/mL浓度范围内线性关系良好, 相关系数r=0.9999, 平均回收率为98.25%, 相对标准偏差(RSD)为1.55%, 表明本法简单准确, 重复性好.

Isolation and Purification of a New Shikimic Acid Derivative from Schisandra Chinensis and Its Content Assay

A new shikimic acid derivative(1) was isolated from the ethanol extract of Schisandra chinensis(Turcz.) Baill. The structure of compound 1 was elucidated as n-butyl shikimate, on the basis of the 1D NMR, HMQC and HMBC spectroscopic techniques. A RP-HPLC method was performed in a extend C18 column with a gradient elution mobile phase of acetonitrile-0.05%H3PO4 water solution at a flow rate of 1.0 mL/min, and the detection wavelength was 215 nm. A good linearity for n-butyl shikimate was obtained within the concentration range of 2—65 μg/mL(r=0.9999), and the recovery was 98.25% with RSD 1.55%. The method was simple, accurate with good repeatability.