A cancer cell turn-on protein-CuSMn nanoparticle as the sensor of breast cancer cell and CH3O-PEG-phosphatide

Cancer activated protein-inorganic nanoparticles can be cancer targeting turn-on imaging and therapy agents. Although various techniques were successfully employed for development of hybrid protein-inorganic nanoparticles, cancer cell activated protein-inorganic nanoparticles have been challengeable. Herein, a cancer cell responsive nanoparticle (PDAMn-CuS@BSA-FA, NPs) was constructed and characterized. It is found that the quenching interaction of dyes (PDA) and central metal in NPs can be adjusted by CH₃O-PEG-phosphatide or the cancer cells, hence, NPs showed turn-on fluorescence emission with the titration of CH₃O-PEG-phosphatide. In particular, breast cancer cells lysis can switch on the green emission greatly, while normal cells show less effect. Breast cancer cells turn-on fluorescence imaging and mitochondria targeting imaging demonstrate that the NPs can sense breast cancer cells and enter mitochondria. Therefore, NPs can be both breast cancer targeting nanosensor.